Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal transgene control in Caenorhabditiselegans.
Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 10.1073/pnas.1720063115.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107130 | pHW522 (Prab-3::cGAL-C::let-858 3'UTR) | cGAL-C driver under the control of C. elegans rab-3 promoter |
| 107131 | pHW530 (Prab-3::cGAL-N::let-858 3'UTR) | cGAL-N driver under the control of C. elegans rab-3 promoter |
| 107132 | pHW533 (Peft-3::cGAL-N::let-858 3'UTR) | cGAL-N driver under the control of C. elegans eft-3 promoter |
| 107133 | pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR) | cGAL-N driver under the control of C. elegans hsp-16.41 promoter |
| 107134 | pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR) | cGAL-N driver under the control of C. elegans myo-2 promoter |
| 107135 | pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR) | cGAL-C driver under the control of C. elegans myo-2 promoter |
| 107136 | pHW531 (Peft-3::cGAL-C::let-858 3'UTR) | cGAL-C driver under the control of C. elegans eft-3 promoter |
| 107137 | pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR) | Dominant negative PKA cGAL effector for C. elegans |
| 107739 | pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR) | mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter |