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NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins.
Campbell AE, Shadle SC, Jagannathan S, Lim JW, Resnick R, Tawil R, van der Maarel SM, Tapscott SJ
Elife. 2018 Mar 13;7. pii: 31023. doi: 10.7554/eLife.31023. PubMed Article

Plasmids from Article

ID Plasmid Purpose
106332pCW57.1-MBD3L2All-in-one lentivector with tet-inducible MBD3L2
106352pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLSCRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus
106353pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLSCRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus
106354pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLSCRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus
106355pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLSCRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus
106357pZLCv2-3xFLAG-dCas9-HA-2xNLSEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

Antibodies from Article