Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition.
Hara M, Lourido S, Petrova B, Lou HJ, Von Stetina JR, Kashevsky H, Turk BE, Orr-Weaver TL
Elife. 2018 Feb 26;7. pii: 33150. doi: 10.7554/eLife.33150. PubMed Article
Hara M, Lourido S, Petrova B, Lou HJ, Von Stetina JR, Kashevsky H, Turk BE, Orr-Weaver TL
Elife. 2018 Feb 26;7. pii: 33150. doi: 10.7554/eLife.33150. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112997 | pGEX6P-1 PUM FL | For protein expression and purification of Drosophila pumilio (amino acids 566-1090) |
| 112998 | pGEX6P-1 BICC FL | For protein expression and purification of full-length Drosophila BicC |
| 112999 | pGEX6P-1 gnu | For protein expression and purification of full-length Drosophila gnu |
| 113001 | pET28b Me31B-3Myc | For protein expression and purification of Myc-tagged full-length Drosophila me31B |
| 113007 | pMAL c2x Tral C 15A | For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A) |
| 113008 | pMAL c2x Tral C | For bacterial expression of MBP fusion of C terminal region of Drosophila Tral |
| 113009 | pMAL c2x Tral N | For bacterial expression of MBP fusion of N terminal region of Drosophila Tral |
| 113010 | pMAL c2x Tral FL-15D | For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations |
| 113011 | pMAL c2x Tral FL-15A | For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A) |
| 113012 | pMAL c2x Tral FL | For bacterial expression of MBP fusion of full-length Drosophila Tral WT |
| 113173 | pFastBac Dual PNG-FLAG PLU-HIS | Baculovirus expression of PNG and PLU |