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Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain.
Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
106432pUBQ10::ZF108_3xFLAG_TET1cdpUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis
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106433pUBQ10::ZF2CACTA1_3xFLAG_TET1cdpUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis
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106434pUBQ10::ZF1CACTA1_3xFLAG_TET1cdpUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis
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106435SunTag-FWAgRNA-4-22aa-TET1cdSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
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106436SunTag-FWAgRNA-4-14aa-TET1cdSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
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106437SunTagCACTA1g2-22aa-TET1cdSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter
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106438SunTagng22aaEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
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106439SunTagng14aaEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
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106441pUBQ10::ZF108_3xFLAG_YPetpUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis
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Antibodies from Article