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Dominant-negative SMARCA4 mutants alter the accessibility landscape of tissue-unrestricted enhancers.
Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland JG, Ku WL, Veverka V, Zhao K, Crabtree GR
Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
PubMed

Plasmids from Article

ID Plasmid Purpose
107056WT Smarca4-sfGFPExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
107057K785R Smarca4-sfGFPExpression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
107058WT Smarca4-V5 IRES-puroExpression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
107059K785R Smarca4-V5 IRES-puroExpression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

Antibodies from Article