A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.
Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander EL, Wilkinson JE, Challen GA, Vakoc CR
Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 10.1016/j.ccell.2017.12.002. PubMed Article
Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander EL, Wilkinson JE, Challen GA, Vakoc CR
Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 10.1016/j.ccell.2017.12.002. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105559 | LEP-shTAF12.364 | retrovirally express of TAF12 shRNA with puro resistance and GFP marker |
| 105560 | LEP-shTAF12.376 | retrovirally express TAF12 shRNA with puro resistance and GFP marker |
| 105561 | LEP-shTAF2.1635 | retrovirally express TAF2 shRNA with puro resistance and GFP marker |
| 105562 | LEP-shTAF2.4392 | retrovirally express TAF2 shRNA with puro resistance and GFP marker |
| 105563 | LEP-shTAF4.2818 | retrovirally express TAF4 shRNA with puro resistance and GFP marker |
| 105564 | LEP-shTAF4.3818 | retrovirally express TAF4 shRNA with puro resistance and GFP marker |
| 105565 | LEP-shMLL-AF9.643 | retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker |
| 105566 | LEP-shMLL-AF9.1039 | retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker |
| 105567 | LEP-shTADA1.1226 | retrovirally express TADA1 shRNA with puro resistance and GFP marker |
| 105568 | LEP-shTADA1.1580 | retrovirally express TADA1 shRNA with puro resistance and GFP marker |
| 105572 | TRIN-E-shTAF12.364 | Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance |
| 105573 | TRIN-E-shTAF12.376 | Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance |
| 105574 | TRIN-shMYB.2572 | Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance |
| 105575 | TRIN-shMYB.2652 | Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance |
| 105577 | MLSE-shTAF12.364 | retrovirally express TAF12 shRNA with GFP marker |
| 105578 | MLSE-shTAF12.376 | retrovirally express TAF12 shRNA with GFP marker |
| 105579 | LENC-shTAF12.364 | retrovirally express TAF12 shRNA with Neo resistance and mCherry marker |
| 105580 | LEP-shREN | retrovirally express control shRNA with puro resistance and GFP marker |
| 105583 | MLSE-shREN | retrovirally express control shRNA with GFP marker |
| 105584 | MLSE-shRPA3.457 | retrovirally express RPA3 shRNA with GFP marker |
| 105585 | TRIN-E-shREN | Dox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance |
| 105586 | TRIN-shREN | Dox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance |
| 105587 | LRG-2.1T-neo-sgROSA | lentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance |
| 105588 | LRG-neo-sgTAF12(mouse)#4.1 | lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance |
| 105590 | LRG-2.1T-neo-sgTAF12(human)#4.4 | lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance |
| 105591 | LRG-2.1T-neo-sgMYB(human)#4.9 | lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance |
| 105592 | LRG-2.1T-neo-sgMYB(human)#5.2 | lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance |
| 105593 | MSCV-PIG-N3FLAG-TAF12 | retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance |
| 105594 | MSCV-PIG-empty | empty control vector with GFP marker and puro resistance |
| 105595 | MSCV-PIG-N3FLAG-TAF12(50-130) | retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance |
| 105596 | MSCV-puro-N3FLAG-MYB-FL | retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance |
| 105597 | MSCV-puro-N3FLAG-MYB-del(TAD) | retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance |
| 105609 | PCDNA3-N3FLAG-MYB-FL | transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells |
| 105610 | PCDNA3-N3FLAG-MYB-DBD(1-200) | transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells |
| 105611 | PCDNA3-N3FLAG-MYB-TAD(251-327) | transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells |
| 105612 | PCDNA3-N3FLAG-MYB-(1-327) | transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells |
| 105613 | PCDNA3-N3HA-TAF12-HFD(50-130) | transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells |
| 105614 | PCDNA3-TAF4(827-909) | transient express TAF4 HFD in HEK 293T cells |
| 105615 | PCDNA3-N3FLAG-TAF12-HFD(50-130) | transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells |
| 105616 | MSCV-PIG-TAF4(827-909) | retrovirally express express mouse TAF4 HFD with GFP marker and puro resistance |
| 105617 | MSCV-PIG-FLAG-TAF4(827-909) | retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance, |
| 105618 | pGEX-4T1-TAF12(50-130) | bacterially express GST tagged TAF12 HFD |
| 105619 | pGEX-4T1-MYB-TAD(251-327) | bacterially express GST tagged MYB TAD |
| 105620 | pET28a-TAF4(827-909) | bacterially express TAF4 HFD |
| 105621 | Lenti-luciferase-P2A-Neo | 3rd generation lentiviral plasmid for expression of luciferase with Neo resistance |
| 105984 | PCDNA3-TADA1-HFD(140-230) | transient express TADA1-HFD fragment in HEK 293T cells |
| 105987 | LRG-2.1T-sgTAF12(human)#4.5 | lentivirally express gRNA targeting human TAF12 HFD with GFP marker |
| 105990 | MSCV-PIG-N3FLAG-TAF10 | retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance |
| 106380 | TtGNN-GFP-empty | dox inducible expression of GFP with neo resistance |
| 106381 | TtGNN-TAF4-HFD | dox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistance |