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CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing.
Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ
Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
PubMed

Plasmids from Article

ID Plasmid Purpose  
102565AsCpf1-2NLSbacterial expression of AsCpf1-2NLS
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102566LbCpf1-2NLSbacterial expression of LbCpf1-2NLS
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107185pT3TS-zAsCpf1plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)
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107187pSP64T-hLbCpf1plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)
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Antibodies from Article