Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks.
Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G
Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 10.1073/pnas.1711979114.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
105242pAP1698-4mammalian eGFP
105243pAP2050-2mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker
105244pAP2042-1mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker
105245pAP1716-1human eGFP with homology arms for Lamin A/C
105246pAP1791-1human eGFP with homology arms for RAB11A (recoded )
105247pAP1892-1human GFP11 with tagRFP 33bp downstream in Lamin A/C
105248pAP1893-1human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)
105249sgPYM1human PYM1 sgRNA
105250pBS-AC3CtermGenomic-mCherrymouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Antibodies from Article