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Abundant off-target edits from site-directed RNA editing can be reduced by nuclear localization of the editing enzyme.

Vallecillo-Viejo IC, Liscovitch-Brauer N, Montiel-Gonzalez MF, Eisenberg E, Rosenthal JJC
RNA Biol. 2018 Jan 2;15(1):104-114. doi: 10.1080/15476286.2017.1387711. Epub 2017 Nov 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172313NLS-4LNDD-pcDNA3.1(+)Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells
172314NLS-4LNDDE488Q-pCDNA3.1(+)Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

Antibodies from Article