Continuous directed evolution of aminoacyl-tRNA synthetases.
Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR
Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. PubMed Article
Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR
Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99147 | pDB007(+) | Accessory plasmid for PACE of MjTyrRS variants |
| 99209 | pDB021CH(+) | Accessory plasmid for PACE of PylRS variants |
| 99210 | pDB038 | Accessory plasmid for PACE of PylRS variants |
| 99211 | pDB038a | Accessory plasmid for PACE of PylRS variants |
| 99212 | pDB038b | Accessory plasmid for PACE of PylRS variants |
| 99214 | pDB007ns2a | Accessory plasmid for PACE of PylRS variants; negative selection |
| 99215 | pDB036a | Accessory plasmid for PACE of MjTyrRS variants; negative selection |
| 99216 | pDB036d | Accessory plasmid for PACE of MjTyrRS variants; negative selection |
| 99217 | pDB023f | Complementary plasmid for aaRS PACE; amber codons in T7 RNAP |
| 99218 | pDB023f1 | Complementary plasmid for aaRS PACE; amber codons in T7 RNAP |
| 99219 | pDB023k | Complementary plasmid for aaRS PACE; amber codons in T7 RNAP |
| 99220 | pDB016 | Accessory plasmid for aaRS PACE; negative selection |
| 99222 | pTECH-chPylRS(IPYE) | Expression plasmid encoding chPylRS(IPYE) and pylT |
| 99397 | pDB070.iodo.5 | Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine |
| 104069 | pTECH-chAcK3RS(IPYE) | Expression plasmid encoding chAcK3RS(IPYE) and pylT |
| 104070 | pTECH-MbAcK3RS(IPYE) | Expression plasmid encoding MbAcK3RS(IPYE) and pylT |
| 104071 | pTECH-MmAcK3RS(IPYE) | Expression plasmid encoding MmAcK3RS(IPYE) and pylT |
| 104072 | pTECH-MbPylRS(IPYE) | Expression plasmid encoding MbPylRS(IPYE) and pylT |
| 104073 | pTECH-MmPylRS(IPYE) | Expression plasmid encoding MmPylRS(IPYE) and pylT |
| 109260 | pDB026a | Accessory plasmid for PACE of MjTyrRS variants |
| 109261 | pDB026e | Accessory plasmid for PACE of MjTyrRS variants |
| 109263 | pDB026g | Accessory plasmid for PACE of MjTyrRS variants |