RNA targeting with CRISPR-Cas13.
Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F
Nature. 2017 Oct 4. doi: 10.1038/nature24049. PubMed Article
Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F
Nature. 2017 Oct 4. doi: 10.1038/nature24049. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 91902 | pC014 - LwCas13a-msfGFP | Expresses active LwCas13a for mammalian RNA targeting |
| 91905 | pC015 - dLwCas13a-NF | Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging |
| 91906 | pC016 - LwCas13a guide expression backbone with U6 promoter | Backbone for cloning LwCas13a guides under U6 promoter |
| 91907 | pC017 - LwCas13a guide expression backbone with tRNAval promoter | Backbone for cloning LwCas13a guides under tRNAval promoter |
| 91908 | pC018 - LshCas13a from Leptotrichia shahii | Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning |
| 91909 | pC019 - LwCas13a from Leptotrichia wadei | Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning |
| 91910 | pC020 - LseCas13a from Listeria seeligeri | Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning |
| 91911 | pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020 | Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning |
| 91912 | pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179 | Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning |
| 91913 | pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710 | Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning |
| 91914 | pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847 | Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning |
| 91915 | pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847 | Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning |
| 91916 | pC026 - PpCas13a from Paludibacter propionicigenes WB4 | Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning |
| 91917 | pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317 | Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning |
| 91918 | pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635 | Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning |
| 91919 | pC029 - Lw2Cas13a from Leptotrichia wadei F0279 | Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning |
| 91920 | pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003 | Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning |
| 91921 | pC031 - RcrCas13a from Rhodobacter capsulatus R121 | Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning |
| 91922 | pC032 - RcdCas13a from Rhodobacter capsulatus DE442 | Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning |
| 91924 | pC034 - LwCas13a-msfGFP-2A-Blast | Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker |
| 91925 | pC035 - dLwCas13a-msfGFP | Expresses catalytically inactive LwCas13a for mammalian RNA targeting |
| 105815 | pC0056 - LwCas13a-msfGFP-NES | Expresses active LwaCas13a-NES for mammalian RNA targeting |
| 107691 | pJJB296 | 35SPromoter-LwaCas13a-HSPTerminator |
| 107694 | pJJB302 | OsActin Promoter-LwaCas13a-HSP Terminator |
| 107699 | pJJB308 | AtU6 Promoter-LwaCas13a-gRNA Scaffold |