New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision.
Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ
J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. PubMed Article
Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ
J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100726 | FKBP-beta2(616-951)-GFP | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100727 | FKBP-beta2(616-951)-GFP Y215A | To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis |
| 100728 | FKBP-beta2(616-951)-GFP ∆CBM | To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis |
| 100729 | FKBP-beta2(616-951)-GFP Double | To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis |
| 100730 | FKBP-beta2(616-951)-mRuby2 | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100731 | FKBP-alpha(740-977)-GFP | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control) |
| 100732 | FKBP-beta1(617-949)-GFP | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100733 | FKBP-epsin(144-575)-GFP | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100734 | FKBP-beta3(702-1094)-GFP | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100735 | FKBP-GTSE1(638-720)-GFP | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100736 | PDZb-beta2(616-951)-mCherry | To express a clathrin "hook" in mammalian cells to hot-wire endocytosis |
| 100738 | CD8-mCherry-FRB | To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis |
| 100739 | CD8-dCherry-FRB | To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N) |
| 100740 | Myc-CD4-mCherry-HA-FRB | To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis |
| 100741 | SH4-FRB-mRFP | To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis |
| 100742 | CD8-pTagRFP657-LOVpep (T406A, T407A, I532A) | To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis |
| 100743 | GST-beta2(616-951) | Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form |
| 100744 | GST-beta2(616-951)Y815A | Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant |
| 100745 | GST-beta2(616-951)∆CBM | Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant |
| 100746 | GST-beta2(616-951)Double | Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant |
| 100748 | MBP-CHC(1-1074)-His6 | Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification. |