Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
Nature. 2017 Sep 20. doi: 10.1038/nature24268. PubMed Article
Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
Nature. 2017 Sep 20. doi: 10.1038/nature24268. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101176 | BPK3258 - human expression plasmid for eSpCas9(1.1) | Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG |
| 101177 | BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1 | Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG |
| 101178 | BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9) | Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG |
| 101179 | MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A | Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG |
| 101180 | MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A | Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG |
| 101181 | MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A | Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG |
| 101182 | MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A | Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG |
| 101183 | MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A | Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG |
| 101184 | MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A | Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG |
| 101185 | MMW2993 - human expression plasmid for SpCas9 Cluster 2 | Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG |
| 101187 | MMW3645 - human expression plasmid for SpCas9 Cluster 3 | Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG |
| 101188 | MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A | Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG |
| 101189 | MMW3770 - human expression plasmid for SpCas9 Cluster 4 | Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG |
| 101190 | MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A | Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG |
| 101191 | BPK4387 - human expression plasmid for SpCas9 Cluster 5 | Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG |
| 101192 | BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A | Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG |
| 101199 | pSHS207 - Bacterial expression plasmid for WT SpCas9 | Bacterial expression plasmid for WT SpCas9 |
| 101200 | pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant | Bacterial expression plasmid for SpCas9, REC2 FRET variant |
| 101201 | pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant | Bacterial expression plasmid for SpCas9, REC3 FRET variant |
| 101202 | pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant | Bacterial expression plasmid for SpCas9∆REC3 variant |
| 101203 | pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant | Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant |
| 101204 | pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant | Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant |
| 101205 | pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain | Bacterial expression plasmid for SpCas9 REC3 domain |
| 101206 | pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant | Bacterial expression plasmid for SpCas9 + Q926A variant |
| 101207 | pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant | Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant |
| 101208 | pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant | Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant |
| 101209 | pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant | Bacterial expression plasmid for SpCas9-HF1 variant |
| 101210 | pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant | Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant |
| 101211 | pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant | Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant |
| 101212 | pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant | Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant |
| 101213 | pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant | Bacterial expression plasmid for SpCas9 + K855A variant |
| 101214 | pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant | Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant |
| 101215 | pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant | Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant |
| 101217 | pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant | Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant |
| 101218 | pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant | Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant |
| 101220 | pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant | Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant |
| 101229 | pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant | Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant |
| 101230 | pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant | Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant |
| 101231 | pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant | Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant |
| 101232 | pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant | Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant |
| 101233 | pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant | Bacterial expression plasmid for SpCas9 Cluster 2 variant |
| 101234 | pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant | Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant |
| 101235 | pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant | Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant |
| 101236 | pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant | Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant |