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RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
Gulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen J
Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8224-E8233. doi: 10.1073/pnas.1712176114. Epub 2017 Sep 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135556pET-28a-His6-SUMO-RABIFExpress human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin
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135557pFastBac HT JS-Rab10wtExpress mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.
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135558pcDNA3.1(+)-RABIF-FLAGExpress human RABIF in mammalian cells, FLAG at N terminal
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135559pcDNA3.1(+)-Rab10-FLAGExpress mouse Rab10 in mammalian cells, FLAG at N terminal
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Antibodies from Article