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Rapid and scalable preparation of bacterial lysates for cell-free gene expression.
Didovyk A, Tonooka T, Tsimring L, Hasty J
ACS Synth Biol. 2017 Aug 10. doi: 10.1021/acssynbio.7b00253.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99244pAD-LyseRFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
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99245pAD-LyseR (in lacZ deficient strain)For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
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99246pAD-GamSExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
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99247LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strainLacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain
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Antibodies from Article