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Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus.

Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99608pSAD-F3-NPEST-iCRE-2A-mCherryPESTSelf-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry
99609pSAD-F3-NPEST-FLPo-2A-mCherryPESTSelf-inactivating DG-Rabies (SiR) expressing FLPo and destabilised mCherry
99610pLenti-CMV-puro-2A-TEVpLentivirus expressing puromycin resistance gene and TEV protease
102984pLenti-CMV-GFP-2A-oG-WPRELentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)
102985pAAV-CMV-FLEX-TVAmCherry-2A-oGAAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
102986pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPREAAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination
102987pLenti-CMV-CAG-GFP-EnvALentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies
104330pAAV-CMV-TVAmCherry-2A-oGAAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)
104332pSAD-F3-NPEST-mCherrySelf-inactivating DG-Rabies (SiR) expressing mCherry

Antibodies from Article