A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.
Alam KK, Tawiah KD, Lichte MF, Porciani D, Burke DH
ACS Synth Biol. 2017 May 26. doi: 10.1021/acssynbio.7b00059. PubMed Article
Alam KK, Tawiah KD, Lichte MF, Porciani D, Burke DH
ACS Synth Biol. 2017 May 26. doi: 10.1021/acssynbio.7b00059. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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87307 | pUC19-T7-SdB-T | Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter. |
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87308 | pUC19-T7-3WJdB-T | Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter. |
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87309 | pUC19-T7-Top-T | Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter. |
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87310 | pUC19-T7-Bottom-T | Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter. |
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87311 | pUC19-P70a-3WJdB-T | Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter. |
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87312 | pUC19-P70a-Top-T | Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter. |
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87313 | pUC19-P70a-Bottom-T | Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter. |
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87314 | pUC19-P70a-Top-T~P70a-Bottom-T | Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters. |
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87315 | pUC19-P70a-Top-T~Bottom-T | Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter. |
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87316 | pUC19-Split-Broccoli-Toehold-Switch | Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry. |
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87317 | pUC19-Trigger-Bottom | Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence. |
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87318 | pUC19-Top-Toehold-mCherry | Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry. |
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