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A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.
Alam KK, Tawiah KD, Lichte MF, Porciani D, Burke DH
ACS Synth Biol. 2017 May 26. doi: 10.1021/acssynbio.7b00059.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87307pUC19-T7-SdB-TExpresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.
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87308pUC19-T7-3WJdB-TExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.
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87309pUC19-T7-Top-TExpresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.
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87310pUC19-T7-Bottom-TExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.
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87311pUC19-P70a-3WJdB-TExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.
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87312pUC19-P70a-Top-TExpresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
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87313pUC19-P70a-Bottom-TExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
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87314pUC19-P70a-Top-T~P70a-Bottom-TExpresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.
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87315pUC19-P70a-Top-T~Bottom-TControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.
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87316pUC19-Split-Broccoli-Toehold-SwitchExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.
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87317pUC19-Trigger-BottomExpresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.
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87318pUC19-Top-Toehold-mCherryExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.
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Antibodies from Article