A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs.
Melero-Fernandez de Mera RM, Li LL, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ
Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89738 | pKCAG-mCherry-OptoJNKi | Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11 |
| 89739 | pmCherry-NES-OptoJNKi | Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11 |
| 89740 | pmCherry-3xNLS-OptoJNKi | Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11 |
| 89741 | pKCAG-H2BmCherry-OptoJNKi | Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11 |
| 89742 | pmCherry-3xNLS-OptoJNKi-lsm | Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11 |
| 89743 | pmCherry-3xNLS-OptoJNKi-dsm | Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11 |
| 89744 | pLuc-OptoJNKi | Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11 |
| 89745 | pLuc-OptoJNKi-dsm | Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11 |
| 89746 | pLuc-OptoJNKi-lsm | Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11 |
| 89747 | pLuc-Optop38i-lsm | Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F |
| 89748 | pLuc-Optop38i-dsm | Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F |
| 89749 | pYpet-Optop38i | Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F |