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Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens.
Morgens DW, Wainberg M, Boyle EA, Ursu O, Araya CL, Tsui CK, Haney MS, Hess GT, Han K, Jeng EE, Li A, Snyder MP, Greenleaf WJ, Kundaje A, Bassik MC
Nat Commun. 2017 May 5;8:15178. doi: 10.1038/ncomms15178.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101926Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancerBassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer. Coexpresses mCherry.
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101927Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatasesBassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.
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101928Bassik lab Human CRISPR-Cas9 Deletion Library - Gene ExpressionBassik lab Human CRISPR-Cas9 Deletion Library - Gene Expression. Coexpresses mCherry.
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101929Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteinsBassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.
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101930Bassik lab Human CRISPR-Cas9 Deletion Library - ProteostasisBassik lab Human CRISPR-Cas9 Deletion Library - Proteostasis. Coexpresses mCherry.
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101931Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motilityBassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility. Coexpresses mCherry.
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101932Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned1Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned1. Coexpresses mCherry.
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101933Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned2Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned2. Coexpresses mCherry.
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101934Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned3Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned3. Coexpresses mCherry.
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Antibodies from Article