A novel method for quantitative measurements of gene expression in single living cells.
Mandic A, Strebinger D, Regali C, Phillips NE, Suter DM
Methods. 2017 May 1;120:65-75. doi: 10.1016/j.ymeth.2017.04.008. Epub 2017 Apr 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115684 | pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro | NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence. |