BLaTM 2.0, a genetic tool revealing preferred antiparallel interaction of transmembrane helix 4 of the dual-topology protein EmrE.
Julius A, Laur L, Schanzenbach C, Langosch D
J Mol Biol. 2017 Apr 18. pii: S0022-2836(17)30166-3. doi: 10.1016/j.jmb.2017.04.003. PubMed Article
Julius A, Laur L, Schanzenbach C, Langosch D
J Mol Biol. 2017 Apr 18. pii: S0022-2836(17)30166-3. doi: 10.1016/j.jmb.2017.04.003. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92060 | C-BLa 2.0 | The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment. |
| 207846 | NBla_1.2_EmrE_TMD4_3P | BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vector |
| 207847 | NBla_1.2_EmrE_TMD4_3P_G90V_G97V | BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vector |
| 207848 | CBla_2.0_EmrE_TMD4_3P | BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector |
| 207849 | CBla_2.0_EmrE_TMD4_3P_G90V_G97V | BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in CBLa 2.0 vector |