Optogenetic control with a photocleavable protein, PhoCl.
Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. PubMed Article
Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87688 | pBAD/HisB-PhoCl | Express PhoCl in E. coli |
| 87689 | pBAD/HisB-PhoCl-MBP | Express PhoCl-MBP in E.coli |
| 87690 | pcDNA-NES-PhoCl-mCherry | Express NES-PhoCl-mCherry in mammalian cells |
| 87691 | pcDNA-NLS-PhoCl-mCherry | Express NLS-PhoCl-mCherry in mammalian cells |
| 87692 | pcDNA-PhoCl-mCherry-myc | Express PhoCl-mCherry with C-term myc tag in mammalian cells |
| 87693 | pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 | Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells |
| 87694 | pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 | Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells |
| 87695 | pUAS-mCherry-NLS | Gal4VP16 driven expression of mCherry-NLS in mammalian cells |
| 87696 | pUAS-NanoLuc | Gal4VP16 driven expression of NanoLuc |
| 87697 | pcDNA-I-PhoCl-HCVp | Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells |
| 87698 | pcDNA-PANX1(HCVp) | Express HCV protease-activatible pannexin-1 in mammalian cells |
| 87699 | pcDNA-mCherry-4A/4B-CAAX | Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells |