Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution.
Mo GC, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J
Nat Methods. 2017 Apr;14(4):427-434. doi: 10.1038/nmeth.4221. Epub 2017 Mar 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87704Dronpa-DEVD-TagRFPTFluorescent biosensor for super-resolution imaging of caspase activity.
Loading...
87705FLINC-AKAR1-CAAXFluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.
Loading...
87706FLINC-AKAR1(TA)-CAAXNegative-control mutant of FLINC-AKAR1-CAAX.
Loading...
87707Lyn-FLINC-EKAR1Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
Loading...
87708Lyn-FLINC-EKAR1(TA)Negative-control mutant of Lyn-FLINC-EKAR1.
Loading...
87709FKBP-DronpaFKBP-tagged Dronpa.
Loading...
87710FRB-TagRFPT-CAAXFRB-tagged TagRFPT; targeted to plasma membrane.
Loading...
87711Dronpa-FHA1Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).
Loading...
87712PKAsub-TagRFPT-CAAXEncodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
Loading...
87740PKAsub(TA)-TagRFPT-CAAXNegative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
Loading...

Antibodies from Article