Ataxin-3 consolidates the MDC1-dependent DNA double-strand break response by counteracting the SUMO-targeted ubiquitin ligase RNF4.
Pfeiffer A, Luijsterburg MS, Acs K, Wiegant WW, Helfricht A, Herzog LK, Minoia M, Bottcher C, Salomons FA, van Attikum H, Dantuma NP
EMBO J. 2017 Mar 8. pii: e201695151. doi: 10.15252/embj.201695151. PubMed Article
Pfeiffer A, Luijsterburg MS, Acs K, Wiegant WW, Helfricht A, Herzog LK, Minoia M, Bottcher C, Salomons FA, van Attikum H, Dantuma NP
EMBO J. 2017 Mar 8. pii: e201695151. doi: 10.15252/embj.201695151. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89975 | GFP-ataxin-3 | Expresses GFP-tagged ataxin-3 |
| 89976 | GFP-ataxin-3 *VBM | Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif |
| 89977 | GFP-ataxin-3 C14A | Expresses catalytic inactive GFP-tagged ataxin-3 |
| 89979 | GFP-ataxin-3 *SIM | Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif |
| 89980 | GFP-ataxin-3 N-t | Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3 |
| 89981 | GFP-ataxin-3 C-t | Expresses the GFP-tagged C-terminus of ataxin-3 |
| 89983 | 10xHis-ataxin-3 *SIM-HA | Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif |