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LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.
Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
96971pMGF130S. pombe lem2 knockout cassette with hygro resistance gene
96972pMGF157ish1-mCherry insertion cassette with Hygro resistance gene
96996pMGF169ish1 mCherry insertion cassette with G418 resistance gene
96997pMGF170ish1-mCherry insertion cassette with NAT resistance gene
96998pMGF172ish1 mCherry insertion cassette with ura4+ gene
96999pMGF173NLS-GFP-lacZ leu1 insertion vector
97005pMGF196Expresses LEMD2-mCherry in mammalian cells under a crippled promoter
97006pMGF182Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

Antibodies from Article