Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis.
Gilbert C, Howarth M, Harwood CR, Ellis T
ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292. PubMed Article
Gilbert C, Howarth M, Harwood CR, Ellis T
ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87370 | pCG-A-sacB(SP)-B | An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly |
| 87371 | pCG-A-celA(SP)-B | An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly |
| 87372 | pCG-A-xynA(SP)-B | An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly |
| 87373 | pCG-B-SpyTag-C | An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly |
| 87374 | pCG-B-mSpyCatcher-C | An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly |
| 87375 | pCG-D-SpyTag-His6-E | An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly |
| 87376 | pCG-D-mSpyCatcher-His6-E | An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass |
| 87377 | pCG004 | ORF part assembly entry vector derived from pHT01. |