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Stepwise assembly of functional C-terminal REST/NRSF transcriptional repressor complexes as a drug target.
Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B, Golas MM
Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 2017 Mar 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
109156pACEBac1-CoRESTflEncodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
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109157pIDS-LSD1flEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system
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109158pIDK-HDAC1flEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system
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109159pACEBAC1-CoREST-CEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
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109160pIDS-LSD1-CEncodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression system
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109161pIDS-RESTwt full-lengthEncodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system
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109162pIDS-RESTmut full-lengthEncodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system
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Antibodies from Article