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One-step generation of conditional and reversible gene knockouts.
Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK
Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84538pUC118-FLIP-Puroa mock vector for generating FLIP targeting vectors
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84539pUC118-FLIP-FlpE-Puroa mock vector for generating FLIP-FlpE targeting vectors
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84540pUC118-mCtnnb1-FLIP-PuroCRISPR - FLIP targeting vector for mouse beta-catenin
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84541pUC118-mCtnnb1-FLIP-FlpE-PuroCRISPR - FLIP-FlpE targeting vector for mouse beta-catenin
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84542gRNA-mCtnnb1mCtnnb1 gRNA expressing vector
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Antibodies from Article