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Model-guided optogenetic study of PKA signaling in budding yeast.
Stewart-Ornstein J, Chen S, Bhatnagar R, Weissman JS, El-Samad H
Mol Biol Cell. 2017 Jan 1;28(1):221-227. doi: 10.1091/mbc.E16-06-0354. Epub 2016 Nov 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
103017pHES609 [prNOp7-PBAC]A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.
103844pHES262 [prADH1-Msn2-mCherry, pnh604]Yeast Expression Plasmid
103846pHES553 [prGal1-RAS2(KAN) pnh604]Yeast Expression Plasmid

Antibodies from Article