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Harnessing Molecular Motors for Nanoscale Pulldown in Live Cells.
Bird JE, Barzik M, Drummond MC, Sutton DC, Goodman SM, Morozko EL, Cole SM, Boukhvalova AK, Skidmore J, Syam D, Wilson EA, Fitzgerald T, Rehman AU, Martin DM, Boger ET, Belyantseva IA, Friedman TB
Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-08-0583.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87255pcDNA3.1-MYO10-HMM-NanotrapExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.
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87256pEGFP-MYO10-HMMExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).
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87257pIZ-EGFP-MYO10-HMMExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).
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89585pEGFP-MYO7A-TAILExpression plasmid for a positive control bait protein in NanoSPD 2.0 assays.
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89586pmCherry-MYRIPExpression plasmid for a positive control prey protein in NanoSPD 2.0 assays.
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Antibodies from Article