Harnessing Molecular Motors for Nanoscale Pulldown in Live Cells.
Bird JE, Barzik M, Drummond MC, Sutton DC, Goodman SM, Morozko EL, Cole SM, Boukhvalova AK, Skidmore J, Syam D, Wilson EA, Fitzgerald T, Rehman AU, Martin DM, Boger ET, Belyantseva IA, Friedman TB
Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-08-0583.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87255 | pcDNA3.1-MYO10-HMM-Nanotrap | Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells. |
| 87256 | pEGFP-MYO10-HMM | Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait). |
| 87257 | pIZ-EGFP-MYO10-HMM | Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait). |
| 89585 | pEGFP-MYO7A-TAIL | Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays. |
| 89586 | pmCherry-MYRIP | Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays. |