Skip to main content

Editing DNA Methylation in the Mammalian Genome.

Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R
Cell. 2016 Sep 22;167(1):233-247.e17. doi: 10.1016/j.cell.2016.08.056. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84475Fuw-dCas9-Tet1CDLentiviral construct to express dCas9 fused with the catalytic domain of Tet1
84476Fuw-dCas9-Dnmt3aLentiviral construct to express dCas9 fused with Dnmt3a
84477pgRNA-modifiedguide RNA expression lentiviral construct
84478Fuw-dCas9-Dnmt3a_IMLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)
84479Fuw-dCas9-Tet1CD_IMLentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)
84569Fuw-dCas9-Dnmt3a-P2A-tagBFPLentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP
84570138-dCas9-Dnmt3aPiggyBac transposon system construct with dCas9-Dnmt3a

Antibodies from Article