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Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.
Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A, Kunieda T
Nat Commun. 2016 Sep 20;7:12808. doi: 10.1038/ncomms12808.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
90019pCXN2KS-DsupTo establish stable line expressing Dsup in constitutive manner
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90020pAcGFP1-N1-DsupTo express GFP-tagged Dsup in mammalian cells transiently
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90021pCold-I-DsupTo express His-tagged Dsup in bacteria
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90022pAcGFP1-N1-Dsup-CTo express GFP-tagged C-terminal region of Dsup in mammalian cells transiently
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90023pAcGFP1-N1-Dsup-deltaCTo express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently
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90024pLKO.1-shDsupTo establish Dsup knock down cells
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Antibodies from Article