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Protecting genome integrity during CRISPR immune adaptation.

Wright AV, Doudna JA
Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 10.1038/nsmb.3289. Epub 2016 Sep 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113248pAW-SpyCas1E. coli expression vector for His-MBP-S. pyogenes Cas1
113249pAW-SpyCas2E. coli expression vector for His-MBP-S. pyogenes Cas2
113250pAW092E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A
113251pAW-SpyCsn2E. coli expression vector for S. pyogenes Csn2-MBP-His
113252pCRISPRpUC19 backbone with E. coli CRISPR array
113253pAW091pUC19 backbone with S. pyogenes CRISPR array

Antibodies from Article