Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity.
Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC
Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632. PubMed Article
Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC
Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121446 | pCDH-puro/Flag PD-L1 WT | expression of huamn PD-L1 wildtype in mammalian cells |
| 121449 | pCDH-puro/Flag PD-L1 N35Q | expression of human PD-L1 mutant in mammalian cells |
| 121451 | pCDH-puro/Flag PD-L1 N200Q | expression of human PD-L1 mutant in mammalian cells |
| 121452 | pCDH-puro/Flag PD-L1 N219Q | expression of huamn PD-L1 mutant in mammalian cells |
| 121453 | pCDH-puro/Flag PD-L1 N192Q/N200Q | expression of human PD-L1 mutant in mammalian cells |
| 121454 | pCDH-puro/Flag PD-L1 N200Q/N219Q | expression of human PD-L1 mutant in mammalian cells |
| 121455 | pCDH-puro/Flag PD-L1 N35/3NQ | expression of human PD-L1 mutant in mammalian cells |
| 121456 | pCDH-puro/Flag PD-L1 4NQ | expression of human PD-L1 mutant in mammalian cells |
| 121466 | pGEX/PD-L1 WT | Expression of GST-human PD-L1 in E. Coli |
| 121467 | pGEX/PD-L1 3SA | Expression of GST-human PD-L1 in E. Coli |
| 121477 | pCDH-Neo/Myc PD-L1 | expression of human Myc-PD-L1 in mamalian cell |
| 121478 | pEGFP-N1/PD-L1 | expression of human PD-L1 in mammalian cells |
| 121479 | pEGFP-N1/PD-L1_ECD | expression of human PD-L1 in mammalian cells |
| 121480 | pEGFP-N1/PD-L1_ICD | expression of human PD-L1 in mammalian cells |
| 121486 | pGIPz-PD-L1 WT | 2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA |
| 121487 | pGIPz-PD-L1 4NQ | Expression of human PD-L1 4NQ with PD-L1 shRNA |
| 121488 | pGIPz-mPD-L1 | Expression of mouse PD-L1 WT |
| 121489 | pGIPz-mPD-L1 3SA | Expression of mouse PD-L1 3SA |
| 121490 | pGIPz-PD-L1 3SA | Expression of human PD-L1 3SA with PD-L1 shRNA |
| 121491 | pCDH-PD-L1 3SA/4NQ | Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA |
| 121492 | pCMV-HA-PD-L1 | Expression of human PD-L1 WT in mammalian cells |
| 121493 | pCMV-HA-PD-L1 3SA | Expression of human PD-L1 3SA in mammalian cells |