Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion.

Ge DT, Tipping C, Brodsky MH, Zamore PD
G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 10.1534/g3.116.032557. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
83954	pCFD4d	Modified from pCFD4, with the vermillion marker and the attB site removed
84005	pCFD4d-U6-1:white1-U6-3:white1	pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter
84006	pUC57-white[coffee]	Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4