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FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions.
Hodgson L, Spiering D, Sabouri-Ghomi M, Dagliyan O, DerMardirossian C, Danuser G, Hahn KM
Nat Chem Biol. 2016 Aug 8. doi: 10.1038/nchembio.2145.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
83359pTriEX-Antenna-GDI Rac1Expression of Antenna-Rac1
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101681pTriEX-Antenna-GDI Cdc42"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
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189669pTriEX-Antenna-GDI RhoAExpression of Antenna-RhoA
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Antibodies from Article