Addgene: Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.

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Timothy Lu
Perez-Pinera et al

Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, Ram R, Lu TK
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
78934	PP255	Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.
78935	PP295	Expresses GFP. Figure 3. Strain 1.
78936	PP296	Expresses GFP. Figure 3. Strain 2.
78937	PP297	Expresses GFP. Figure 3. Strain 3.
78938	PP298	Expresses GFP. Figure 3. Strain 4.
78939	PP299	Expresses GFP. Figure 3. Strain 6.
78940	PP300	Expresses GFP. Figure 3. Strain 7.
78941	PP318	Expresses GFP. Figure 3. Strain 5.
78942	PP228	Expresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal Promoter: mCYC.
78943	PP322	Expresses GFP. Figure 3. Strain 9.
78944	PP74	Supplemental Figure 3. Integration Site 1.
78945	PP75	Supplemental Figure 3. Integration Site 2.
78946	PP76	Supplemental Figure 3. Integration Site 3.
78947	PP77	Supplemental Figure 3. Integration Site 4.
78948	PP78	Supplemental Figure 3. Integration Site 5.
78949	PP79	Supplemental Figure 3. Integration Site 6.
78950	PP69	Supplemental Figure 3. Integration Site 7.
78951	PP67	Supplemental Figure 3. Integration Site 8.
78952	PP151	Supplemental Figure 3. Integration Site 9.
78953	PP43	Figure 2. Expresses BxbI.
78954	PP44	Figure 2. Expresses R4.
78955	PP45	Figure 2. Expresses TP901-1.
78961	PP259	Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP.
78962	PP258	Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP.
78963	PP257	Promoter: GAP, minimal Promoter: mCYC. Figure 4. Expresses GFP.
78964	PP247	Promoter: scTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.
78965	PP245	Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.
78966	PP246	Promoter: GAP6, minimal Promoter: AOX1. Figure 4. Expresses GFP.
78967	PP277	Promoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.
78968	PP275	Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.
78969	PP190	Promoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.
78970	PP276	Promoter: GAP6, minimal Promoter: GCW14. Figure 4. Expresses GFP.
78971	PP273	Promoter: ppTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.
78972	PP263	Promoter: scTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.
78973	PP261	Promoter: GAP, minimal Promoter: GAP6. Figure 4. Expresses GFP.
78974	PP262	Promoter: GAP6, minimal Promoter: GAP6. Figure 4. Expresses GFP.
78975	PP254	Promoter: GAP6, minimal Promoter: GAP. Figure 4. Expresses GFP.
78976	PP253	Promoter: GAP, minimal Promoter: GAP. Figure 4. Expresses GFP.
78977	PP229	Promoter: ppTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.
78978	PP244	Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.
78979	PP310	245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.
78980	PP311	246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.
78981	PP362	255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.
78982	PP324	246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.
78983	PP326	245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.
78984	PP363	255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.
78985	PP165	Supplemental Figure 4. Promoter GCW14. Expresses GFP.
78986	PP152	Supplemental Figure 4. Promoter long ppTEF1. Expresses GFP.
78987	PP87	Supplemental Figure 4. Promoter scTEF1. Expresses GFP.
78988	PP164	Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.
78989	PP149	Supplemental Figure 4. Promoter WT GAP. Expresses GFP.
78990	PP93	Supplemental Figure 4. No Promoter.
78991	PP154	Supplemental Figure 4. Promoter GAP1. Expresses GFP.
78992	PP160	Supplemental Figure 4. Promoter GAP2. Expresses GFP.
78993	PP161	Supplemental Figure 4. Promoter GAP3. Expresses GFP.
78994	PP155	Supplemental Figure 4. Promoter GAP4. Expresses GFP.
78995	PP162	Supplemental Figure 4. Promoter GAP5. Expresses GFP.
78996	PP163	Supplemental Figure 4. Promoter GAP6. Expresses GFP.
78997	PP153	Supplemental Figure 4. Promoter GAP7. Expresses GFP.

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