Sensitivity and engineered resistance of myeloid leukemia cells to BRD9 inhibition.
Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G, McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, Suzuki Y, Koegl M, Vakoc CR
Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115. PubMed Article
Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G, McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, Suzuki Y, Koegl M, Vakoc CR
Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75114 | MSCV-BRD9-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding wild type human BRD9 |
| 75115 | MSCV-BRD9_dBD-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain |
| 75116 | MSCV-BRD9_N216A-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation |
| 75118 | MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain |
| 75119 | MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain |
| 75120 | MSCV-BRD9_BET-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 |
| 75121 | MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain |
| 75122 | MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag |
| 75123 | MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag |
| 75124 | MSCV-Myc-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding wild type murine Myc |
| 75125 | MSCV-EZH2-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding wild type human EZH2 |
| 75126 | MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP | Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain |
| 75129 | MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP | Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9) |
| 75130 | MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP | Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9) |
| 75132 | MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry | Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9) |
| 75133 | MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry | Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9) |
| 75135 | MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR | Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9) |
| 75136 | MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR | Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9) |
| 75138 | MSCV-miRE_shBRD9_508-SV40-GFP | Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD) |
| 75139 | MSCV-miRE_shBRD9_561-SV40-GFP | Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD) |