A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of synapses.
Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, Sanes JR, Ting AY
Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563. PubMed Article
Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, Sanes JR, Ting AY
Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73143 | CAG sHRPa-NRX | Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter. |
| 73144 | CAG sHRPb-NLG | Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter. |
| 73145 | CAG sHRPa-FRB-pre mGRASP | Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4. |
| 73146 | CAG sHRPb-FKBP-post mGRASP | Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted. |
| 73147 | Synapsin sHRPa-NRX in FSW lentiviral vector | Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter. |
| 73148 | Synapsin sHRPb-NLG in FSW lentiviral vector | Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter. |
| 73149 | pDisplay sHRPa-KDEL | Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter. |
| 73150 | pDisplay sHRPb-KDEL | Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter. |
| 73151 | YIP sHRPa-Aga1P | Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome. |
| 73152 | pCTCON2 Aga2P-HRP | Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression. |
| 79910 | pXL-CAG-ICeuI-sHRPaNRX3beta | piggyBac transposon vector, sHRPaNRX3beta |
| 79912 | pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1 | piggyBac transposon vector, Cerulean-2A-sHRPbNLG1 |
| 79913 | pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta | piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta |
| 79914 | pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1 | piggyBac transposon vector, Zeocin-2A-sHRPbNLG1 |