Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, Savage DF
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. PubMed Article
Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, Savage DF
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74490 | pBLO1811_Cas9_noNLS_human | Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA |
| 74491 | pBLO1808_arC9_2xNLS_human | Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA |
| 74493 | pBLO1806_Cas9_2xNLS_human | Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA |
| 74494 | pBLO1811_arC9_noNLS_human | Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA |
| 117170 | pBC2101-reci | U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS |
| 117171 | pBC2102-reci | U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS |
| 117172 | pBC2103-reci | U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS |
| 121418 | pBLO5.01 | pUC19_BsaI-dCas9-BsaI |
| 121495 | pBLO 4.01 | engineered Mu transposon |
| 121496 | pBLO 9.2_bCas9-CP199 | TetR_tetR/tetA promoters_dCas9-CP_CM_P15A |
| 121497 | pBLO 9.3_bCas9-CP230 | TetR_tetR/tetA promoters_dCas9-CP_CM_P15A |
| 121498 | pBLO 9.6_bCas9-CP1010 | TetR_tetR/tetA promoters_dCas9-CP_CM_P15A |
| 121500 | pBLO 9.15_bCas9-CP1249 | TetR_tetR/tetA promoters_dCas9-CP_CM_P15A |
| 121501 | pBLO 9.16_bCas9-CP1282 | TetR_tetR/tetA promoters_dCas9-CP_CM_P15A |
| 121624 | pBLO 36.02_ProCas9TEV | TetR_tetR/tetA promoters_bCas9-CP199-TEVlinker-2xflag_CM_P15A |
| 121629 | pBLO43.3_human ProCas9Flavi | U6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1 |
| 121630 | pBLO43.3.-6_human ProCas9Flavi-6 | U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1 |
| 121631 | pBLO44.1_dProtease | CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1 |
| 121632 | pBLO44.3_TuMVpro | CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1 |
| 121633 | pBLO44.4_PPVpro | CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1 |
| 121634 | pBLO44.5_PVYpro | CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1 |
| 121635 | pBLO44.6_ZIKVpro | CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1 |
| 121638 | pBLO44.7_WNVpro | CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1 |