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Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes.
Morgan B, Van Laer K, Owusu TN, Ezerina D, Pastor-Flores D, Amponsah PS, Tursch A, Dick TP
Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. Epub 2016 Apr 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
83238p415 TEF roGFP2‐Tsa2ΔCRThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
83239p415 TEF roGFP2‐Tsa2ΔCPΔCRThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
83240p415 TEF Su9‐roGFP2‐Tsa2ΔCRThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.

Antibodies from Article