Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
An improved smaller biotin ligase for BioID proximity labeling.
Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ
Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. PubMed Article

Plasmids from Article

ID Plasmid Purpose
74223myc-BioID2-MCSTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
74224MCS-BioID2-HATo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
80899MCS-13X Linker-BioID2-HATo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
80900mycBioID2-pBABE-puroTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tag
92308mycBioID2-13X Linker-MCSTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

Antibodies from Article