SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.
Schwartz ML, Jorgensen EM
Genetics. 2016 Feb 2. pii: genetics.115.184275. PubMed Article
Schwartz ML, Jorgensen EM
Genetics. 2016 Feb 2. pii: genetics.115.184275. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73682 | pMLS234 | SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker |
| 73683 | pMLS235 | SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker |
| 73684 | pMLS236 | SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker |
| 73685 | pMLS260 | Punc-17::2xNLS-FLP-D5 Expression vector |
| 73687 | pMLS360 | Punc-47::2xNLS-FLP-D5 Expression vector |
| 73714 | pMLS134 | SapTrap destination vector for building sgRNA expression vectors. |
| 73715 | pMLS256 | SapTrap destination vector for building combined sgRNA expression + repair template vectors |
| 73716 | pMLS257 | SapTrap destination vector for building repair template only vectors |
| 73717 | pMLS328 | C. elegans germline CRE expression vector |
| 73718 | pMLS262 | C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector |
| 73719 | pMLS338 | SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9 |
| 73720 | pMLS252 | SapTrap GFP + Cbr-unc-119 donor plasmid |
| 73721 | pMLS254 | SapTrap Halotag + Cbr-unc-119 donor plasmid |
| 73722 | pMLS271 | SapTrap SNAPtag + Cbr-unc-119 donor plasmid |
| 73723 | pMLS286 | Saptrap tagRFP + Cbr-unc-119 donor plasmid |
| 73724 | pMLS291 | SapTrap mCherry + Cbr-unc-119 donor plasmid |
| 73725 | pMLS292 | SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid |
| 73726 | pMLS268 | SapTrap SL2 C-terminal connector donor plasmid |
| 73727 | pMLS269 | SapTrap SL2::SV40-NLS C-terminal connector donor plasmid |
| 73728 | pMLS272 | SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid |
| 73729 | pMLS279 | SapTrap FLP-on C-terminal connector donor plasmid |
| 73730 | pMLS287 | SapTrap flexible linker C-terminal connector donor plasmid |
| 73731 | pMLS382 | SapTrap Flagtag-TEV C-terminal connector donor plasmid |
| 73732 | pMLS281 | SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid |
| 73733 | pMLS282 | SapTrap FLP-on N-terminal connector donor plasmid |
| 73734 | pMLS285 | SapTrap egl-13 NLS connector donor plasmid |
| 73735 | pMLS288 | SapTrap flexible linker N-terminal connector donor plasmid |
| 73736 | pMLS383 | SapTrap TEV-Flagtag N-terminal connector donor plasmid |
| 73737 | pMLS297 | SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid |
| 73738 | pMLS280 | Simple cloning vector |
| 73739 | pWD509 | Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5 |