A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo.
Polka JK, Silver PA
ACS Synth Biol. 2016 Feb 3. PubMed Article
Polka JK, Silver PA
ACS Synth Biol. 2016 Feb 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71224 | Reb_1 aka PAS619 | Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone |
| 71225 | Reb_79 aka PAS620 | Reb_1 with RebA(Q91R) |
| 71226 | Reb_80 aka PAS621 | Reb_1 with RebB(I67T, N84I) |
| 71227 | Reb_81 aka PAS622 | Reb_1 with RebB(T28A, D72G) |
| 71228 | Reb_82 aka PAS623 | Reb_1 with RebB(S82P, D88G) |
| 71229 | Reb_83 aka PAS624 | Reb_1 with RebA(G53A, D89G) |
| 71230 | Reb_140 aka PAS625 | Reb_1 with RebA(S88P) |
| 71231 | Reb_65 aka PAS626 | Reb_1 with RebA(Y82A) |
| 71232 | Reb_67 aka PAS627 | Reb_1 with RebA(S88A) |
| 71233 | Reb_221 aka PAS628 | Reb_1 with RebA(G90A) |
| 71234 | Reb_206 aka PAS629 | Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry |
| 71235 | Reb_207 aka PAS630 | Reb_206 with RebA(S88P) |