Conformational control of DNA target cleavage by CRISPR-Cas9.
Sternberg SH, LaFrance B, Kaplan M, Doudna JA
Nature. 2015 Nov 5;527(7576):110-3. doi: 10.1038/nature15544. Epub 2015 Oct 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87346 | pSHS212 | Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid |
| 87348 | pSHS293 | D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct |
| 87350 | pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant | S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct |
| 87354 | pSHS248 | S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct |