Using the GEMM-ESC strategy to study gene function in mouse models.
Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. Epub 2015 Oct 15. PubMed Article
Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. Epub 2015 Oct 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63575 | pCol1a1-frt (FVB) | Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB. |
| 63576 | pFrt-invCAG-ires-Luc | Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites. |
| 63577 | pFrt-invCAG-Luc | Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites |