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The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.

Reisch CR, Prather KL
Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62654pKDsgRNA-ackArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression
62655pCas9-CR4Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR
62656pKDsgRNA-p15Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Antibodies from Article