Addgene: An Alternative Method to Facilitate cDNA Cloning for Expression Studies in Mammalian Cells by Introducing Positive Blue White Selection in Vaccinia Topoisomerase I-Mediated Recombination.

Enter a distinct sequence that is an important, differentiating feature. For example, the coding region of a gene, instead of the plasmid origin of replication.

Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory. Visit the corresponding plasmid webpage to view additional details about a matching plasmid.

If no results are returned:

Try a different isoform or region of the desired sequence.
Choose a different BLAST database. Try the general “All Addgene Plasmids” (default selection), instead of a specific database, such as “Plant Expression Plasmids”
Try selecting a different BLAST algorithm:
- megablast: Designed for comparing sequences within the same, or closely related, species. Default selection.
- blastn: Designed for comparing sequences from different species. May return additional results, if exact species match is not required.
- blastn-short: Optimized for searching with shorter sequences (<= 30 nucleotides) but can still be effective with slightly larger sequences.
- tblastn: Designed for comparing protein sequences against a translated nucleotide sequence database. Helpful for finding plasmids with codon-optimized sequences.
- tblastn-fast: A faster version of tblastn that may return results more quickly, but is less sensitive
There may not be a match in our database.

You can adjust the Max Results setting on the results page from 25 to 500. If many sequences share the same top E-value, only a truncated set of equally high-scoring matches will be shown. Set the Max Results to 500 to see more matches.