CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM
Genome Res. 2015 Sep 9.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63934 | pFETCh_Donor (EMM0021) | 3X Flag with P2A-Neo for 3' tagging of endogenous proteins |
| 64036 | PX458_CEBPB_1 | Expresses gRNA against human CEBPB along with Cas9 with 2A GFP |
| 64047 | PX458_CEBPB_2 | Expresses gRNA against human CEBPB along with Cas9 with 2A GFP |
| 64055 | pFETCh_CEBPB | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CEBPB |
| 64056 | pFETCh_RAD21 | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21 |
| 64057 | PX458_RAD21_1 | Expresses gRNA against human RAD21 along with Cas9 with 2A GFP |
| 64253 | pFETCh_GABPA | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA |
| 64254 | PX458_GABPA_1 | Expresses gRNA against human GABPA along with Cas9 with 2A GFP |
| 64255 | PX458_GABPA_2 | Expresses gRNA against human GABPA along with Cas9 with 2A GFP |
| 64689 | pFETCH_ATF1 | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1 |
| 64690 | PX458_ATF1_1 | Expresses gRNA against human ATF1 along with Cas9 with 2A GFP |
| 64938 | pFETCH_CREB1 | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1 |
| 64939 | PX458_CREB1_1 | Expresses gRNA against human CREB1 along with Cas9 with 2A GFP |
| 64940 | PX458_CREB1_2 | Expresses gRNA against human CREB1 along with Cas9 with 2A GFP |
| 86260 | pFETCh_KDM1A | Donor vector for 3' FLAG tag of human KDM1A |
| 86261 | pFETCh_FOXP1 | Donor vector for 3' FLAG tag of human FOXP1 |
| 86262 | pFETCh_HLF | Donor vector for 3' FLAG tag of human HLF |
| 86263 | pFETCh_MBD1_iso1 | Donor vector for 3' FLAG tag of human MBD1_iso1 |
| 86264 | pFETCh_ZNF644 | Donor vector for 3' FLAG tag of human ZNF644 |
| 86265 | pFETCh_KDM6A | Donor vector for 3' FLAG tag of human KDM6A |
| 86266 | pFETCh_KAT7 | Donor vector for 3' FLAG tag of human KAT7 |
| 86267 | pFETCh_SAP130 | Donor vector for 3' FLAG tag of human SAP130 |
| 86268 | pFETCh_ZSCAN9 | Donor vector for 3' FLAG tag of human ZSCAN9 |
| 86269 | pFETCh_KLF11 | Donor vector for 3' FLAG tag of human KLF11 |
| 86270 | pFETCh_ZNF652 | Donor vector for 3' FLAG tag of human ZNF652 |
| 86271 | pFETCh_HMG20A | Donor vector for 3' FLAG tag of human HMG20A |
| 86272 | pFETCh_HBP1 | Donor vector for 3' FLAG tag of human HBP1 |
| 86274 | pFETCh_MIER3 | Donor vector for 3' FLAG tag of human MIER3 |
| 86276 | pFETCh_ZGPAT | Donor vector for 3' FLAG tag of human ZGPAT |
| 86277 | pFETCh_MLX | Donor vector for 3' FLAG tag of human MLX |
| 86278 | pFETCh_ZNF580 | Donor vector for 3' FLAG tag of human ZNF580 |
| 86279 | pFETCh_GATAD2A | Donor vector for 3' FLAG tag of human GATAD2A |
| 86280 | pFETCh_MIXL1 | Donor vector for 3' FLAG tag of human MIXL1 |
| 86281 | pFETCh_TEAD3 | Donor vector for 3' FLAG tag of human TEAD3 |
| 86282 | pFETCh_ZNF792 | Donor vector for 3' FLAG tag of human ZNF792 |
| 86283 | pFETCh_NCoA2 | Donor vector for 3' FLAG tag of human NCoA2 |
| 86284 | pFETCh_NR2F6 | Donor vector for 3' FLAG tag of human NR2F6 |
| 86285 | pFETCh_CEBPG | Donor vector for 3' FLAG tag of human CEBPG |
| 86286 | pFETCh_KLF9 | Donor vector for 3' FLAG tag of human KLF9 |
| 86287 | pFETCh_NFIL3 | Donor vector for 3' FLAG tag of human NFIL3 |
| 86288 | pFETCh_ARID5B | Donor vector for 3' FLAG tag of human ARID5B |
| 86289 | pFETCh_HOMEZ | Donor vector for 3' FLAG tag of human HOMEZ |
| 86291 | pFETCh_TEAD1 | Donor vector for 3' FLAG tag of human TEAD1 |
| 86292 | pFETCh_SOX5 | Donor vector for 3' FLAG tag of human SOX5 |
| 86293 | pFETCh_CEBPA | Donor vector for 3' FLAG tag of human CEBPA |
| 86294 | pFETCh_ZFP1_iso1 | Donor vector for 3' FLAG tag of human ZFP1_iso1 |
| 86295 | pFETCh_SP5 | Donor vector for 3' FLAG tag of human SP5 |
| 86296 | pFETCh_RFXANK | Donor vector for 3' FLAG tag of human RFXANK |
| 86297 | PX458_KDM1A_1 | Encodes gRNA for 3' target of human KDM1A |
| 86298 | PX458_KDM1A_2 | Encodes gRNA for 3' target of human KDM1A |
| 86299 | PX458_FOXP1_1 | Encodes gRNA for 3' target of human FOXP1 |
| 86300 | PX458_FOXP1_2 | Encodes gRNA for 3' target of human FOXP1 |
| 86301 | PX458_HLF_1 | Encodes gRNA for 3' target of human HLF |
| 86302 | PX458_HLF_2 | Encodes gRNA for 3' target of human HLF |
| 86303 | PX458_MBD1_iso1_1 | Encodes gRNA for 3' target of human MBD1_iso1 |
| 86304 | PX458_MBD1_iso1_2 | Encodes gRNA for 3' target of human MBD1_iso1 |
| 86305 | PX458_ZNF644_1 | Encodes gRNA for 3' target of human ZNF644 |
| 86306 | PX458_ZNF644_2 | Encodes gRNA for 3' target of human ZNF644 |
| 86307 | PX458_KDM6A_1 | Encodes gRNA for 3' target of human KDM6A |
| 86308 | PX458_KDM6A_2 | Encodes gRNA for 3' target of human KDM6A |
| 86309 | PX458_KAT7_1 | Encodes gRNA for 3' target of human KAT7 |
| 86310 | PX458_SAP130_1 | Encodes gRNA for 3' target of human SAP130 |
| 86311 | PX458_SAP130_2 | Encodes gRNA for 3' target of human SAP130 |
| 86312 | PX458_ZSCAN9_1 | Encodes gRNA for 3' target of human ZSCAN9 |
| 86313 | PX458_ZSCAN9_2 | Encodes gRNA for 3' target of human ZSCAN9 |
| 86314 | PX458_KLF11_1 | Encodes gRNA for 3' target of human KLF11 |
| 86315 | PX458_KLF11_2 | Encodes gRNA for 3' target of human KLF11 |
| 86316 | PX458_ZNF652_1 | Encodes gRNA for 3' target of human ZNF652 |
| 86317 | PX458_ZNF652_2 | Encodes gRNA for 3' target of human ZNF652 |
| 86318 | PX458_HMG20A_1 | Encodes gRNA for 3' target of human HMG20A |
| 86319 | PX458_HMG20A_2 | Encodes gRNA for 3' target of human HMG20A |
| 86320 | PX458_HBP1_1 | Encodes gRNA for 3' target of human HBP1 |
| 86321 | PX458_KDM3A_1 | Encodes gRNA for 3' target of human KDM3A |
| 86322 | PX458_KDM3A_2 | Encodes gRNA for 3' target of human KDM3A |
| 86323 | PX458_MIER3_1 | Encodes gRNA for 3' target of human MIER3 |
| 86324 | PX458_MIER3_2 | Encodes gRNA for 3' target of human MIER3 |
| 86325 | PX458_TFE3_1 | Encodes gRNA for 3' target of human TFE3 |
| 86326 | PX458_TFE3_2 | Encodes gRNA for 3' target of human TFE3 |
| 86327 | PX458_ZGPAT_1 | Encodes gRNA for 3' target of human ZGPAT |
| 86328 | PX458_ZGPAT_2 | Encodes gRNA for 3' target of human ZGPAT |
| 86329 | PX458_MLX_1 | Encodes gRNA for 3' target of human MLX |
| 86330 | PX458_MLX_2 | Encodes gRNA for 3' target of human MLX |
| 86331 | PX458_ZNF580_1 | Encodes gRNA for 3' target of human ZNF580 |
| 86332 | PX458_ZNF580_2 | Encodes gRNA for 3' target of human ZNF580 |
| 86333 | PX458_GATAD2A_1 | Encodes gRNA for 3' target of human GATAD2A |
| 86334 | PX458_GATAD2A_2 | Encodes gRNA for 3' target of human GATAD2A |
| 86335 | PX458_MIXL1_1 | Encodes gRNA for 3' target of human MIXL1 |
| 86336 | PX458_MIXL1_2 | Encodes gRNA for 3' target of human MIXL1 |
| 86337 | PX458_TEAD3_1 | Encodes gRNA for 3' target of human TEAD3 |
| 86338 | PX458_TEAD3_2 | Encodes gRNA for 3' target of human TEAD3 |
| 86339 | PX458_ZNF792_1 | Encodes gRNA for 3' target of human ZNF792 |
| 86340 | PX458_ZNF792_2 | Encodes gRNA for 3' target of human ZNF792 |
| 86341 | PX458_NCoA2_1 | Encodes gRNA for 3' target of human NCoA2 |
| 86342 | PX458_NR2F6_1 | Encodes gRNA for 3' target of human NR2F6 |
| 86343 | PX458_NR2F6_2 | Encodes gRNA for 3' target of human NR2F6 |
| 86344 | PX458_CEBPG_1 | Encodes gRNA for 3' target of human CEBPG |
| 86345 | PX458_CEBPG_2 | Encodes gRNA for 3' target of human CEBPG |
| 86346 | PX458_KLF9_1 | Encodes gRNA for 3' target of human KLF9 |
| 86347 | PX458_KLF9_2 | Encodes gRNA for 3' target of human KLF9 |
| 86348 | PX458_NFIL3_1 | Encodes gRNA for 3' target of human NFIL3 |
| 86349 | PX458_NFIL3_2 | Encodes gRNA for 3' target of human NFIL3 |
| 86350 | PX458_ARID5B_1 | Encodes gRNA for 3' target of human ARID5B |
| 86351 | PX458_ARID5B_2 | Encodes gRNA for 3' target of human ARID5B |
| 86352 | PX458_HOMEZ_1 | Encodes gRNA for 3' target of human HOMEZ |
| 86353 | PX458_HOMEZ_2 | Encodes gRNA for 3' target of human HOMEZ |
| 86354 | PX458_DMAP1_1 | Encodes gRNA for 3' target of human DMAP1 |
| 86355 | PX458_DMAP1_2 | Encodes gRNA for 3' target of human DMAP1 |
| 86356 | PX458_TEAD1_1 | Encodes gRNA for 3' target of human TEAD1 |
| 86357 | PX458_SOX5_1 | Encodes gRNA for 3' target of human SOX5 |
| 86358 | PX458_CEBPA_1 | Encodes gRNA for 3' target of human CEBPA |
| 86359 | PX458_CEBPA_2 | Encodes gRNA for 3' target of human CEBPA |
| 86360 | PX458_ZFP1_iso1_1 | Encodes gRNA for 3' target of human ZFP1_iso1 |
| 86361 | PX458_ZFP1_iso1_2 | Encodes gRNA for 3' target of human ZFP1_iso1 |
| 86362 | PX458_SP5_1 | Encodes gRNA for 3' target of human SP5 |
| 86363 | PX458_SP5_2 | Encodes gRNA for 3' target of human SP5 |
| 86364 | PX458_RFXANK_1 | Encodes gRNA for 3' target of human RFXANK |
| 86365 | PX458_RFXANK_2 | Encodes gRNA for 3' target of human RFXANK |
| 101069 | pFETCh_RERE | Donor vector for 3' FLAG tag of human RERE |
| 101070 | pFETCh_KMT2B | Donor vector for 3' FLAG tag of human KMT2B |
| 101071 | pFETCh_AHR | Donor vector for 3' FLAG tag of human AHR |
| 101072 | pFETCh_ARID4B | Donor vector for 3' FLAG tag of human ARID4B |
| 101073 | PX458_RERE_1 | Encodes gRNA for 3' target of human RERE |
| 101074 | PX458_KMT2B_1 | Encodes gRNA for 3' target of human KMT2B |
| 101075 | PX458_KMT2B_2 | Encodes gRNA for 3' target of human KMT2B |
| 101076 | PX458_AHR_1 | Encodes gRNA for 3' target of human AHR |
| 101077 | PX458_AHR_2 | Encodes gRNA for 3' target of human AHR |
| 101078 | PX458_ARID4B_1 | Encodes gRNA for 3' target of human ARID4B |
| 104035 | pFETCh_GABPB1_iso2 | Donor vector for 3' FLAG tag of human GABPB1_iso2 |
| 104036 | pFETCh_GATAD1 | Donor vector for 3' FLAG tag of human GATAD1 |
| 104037 | pFETCh_ZNF511 | Donor vector for 3' FLAG tag of human ZNF511 |
| 104038 | pFETCh_BCL6_iso1 | Donor vector for 3' FLAG tag of human BCL6_iso1 |
| 104039 | pFETCh_NFIA_iso1 | Donor vector for 3' FLAG tag of human NFIA_iso1 |
| 104040 | pFETCh_RXRB | Donor vector for 3' FLAG tag of human RXRB |
| 104041 | PX458_GABPB1_iso2_1 | Encodes gRNA for 3' target of human GABPB1_iso2 along with Cas9 with 2A GFP |
| 104042 | PX458_GATAD1_1 | Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP |
| 104043 | PX458_GATAD1_2 | Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP |
| 104044 | PX458_ZNF511_1 | Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP |
| 104045 | PX458_ZNF511_2 | Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP |
| 104046 | PX458_BCL6_iso1_1 | Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP |
| 104047 | PX458_BCL6_iso1_2 | Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP |
| 104048 | PX458_NFIA_iso1_1 | Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP |
| 104049 | PX458_NFIA_iso1_2 | Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP |
| 104050 | PX458_RXRB_1 | Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP |
| 104051 | PX458_RXRB_2 | Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP |
| 128235 | pFETCh_ZBTB1 | Donor vector for 3' FLAG tag of human ZBTB1 |
| 128236 | pFETCh_ZNF326_iso1 | Donor vector for 3' FLAG tag of human ZNF326_iso1 |
| 128238 | pFETCh_SIX4_iso1 | Donor vector for 3' FLAG tag of human SIX4_iso1 |
| 128239 | pFETCh_ZHX3_iso1 | Donor vector for 3' FLAG tag of human ZHX3_iso1 |
| 128240 | pFETCh_ZBTB49_iso1 | Donor vector for 3' FLAG tag of human ZBTB49_iso1 |
| 128241 | pFETCh_SSX2B | Donor vector for 3' FLAG tag of human SSX2B |
| 128242 | PX458_ZBTB1_1 | Encodes gRNA for 3' target of human ZBTB1 |
| 128243 | PX458_ZNF326_iso1_1 | Encodes gRNA for 3' target of human ZNF326_iso1 |
| 128244 | PX458_ZNF326_iso1_2 | Encodes gRNA for 3' target of human ZNF326_iso1 |
| 128245 | PX458_THRA_iso1_1 | Encodes gRNA for 3' target of human THRA_iso1 |
| 128246 | PX458_THRA_iso1_2 | Encodes gRNA for 3' target of human THRA_iso1 |
| 128247 | PX458_SIX4_iso1_1 | Encodes gRNA for 3' target of human SIX4_iso1 |
| 128248 | PX458_SIX4_iso1_2 | Encodes gRNA for 3' target of human SIX4_iso1 |
| 128249 | PX458_ZHX3_iso1_1 | Encodes gRNA for 3' target of human ZHX3_iso1 |
| 128250 | PX458_ZBTB49_iso1_1 | Encodes gRNA for 3' target of human ZBTB49_iso1 |
| 128251 | PX458_ZBTB49_iso1_2 | Encodes gRNA for 3' target of human ZBTB49_iso1 |
| 128252 | PX330_SSX2B | Encodes gRNA for 3' target of human SSX2B |
| 135726 | pFETCh_ZNF331 | Donor vector for 3' FLAG tag of human ZNF331 |
| 135727 | pFETCh_ZBTB21 | Donor vector for 3' FLAG tag of human ZBTB21 |
| 135728 | pFETCh_CIC_iso1 | Donor vector for 3' FLAG tag of human CIC_iso1 |
| 135729 | pFETCh_MLLT10_iso1 | Donor vector for 3' FLAG tag of human MLLT10_iso1 |
| 135730 | pFETCh_ZBTB7B | Donor vector for 3' FLAG tag of human ZBTB7B |
| 135731 | pFETCh_ZNF264_iso1 | Donor vector for 3' FLAG tag of human ZNF264_iso1 |
| 135732 | pFETCh_MAF1_iso1 | Donor vector for 3' FLAG tag of human MAF1_iso1 |
| 135733 | pFETCh_MED13_iso1 | Donor vector for 3' FLAG tag of human MED13_iso1 |
| 135734 | pFETCh_FOXM1_iso1 | Donor vector for 3' FLAG tag of human FOXM1_iso1 |
| 135735 | pFETCh_ZNF503 | Donor vector for 3' FLAG tag of human ZNF503 |
| 135737 | pFETCh_ELK1_iso1 | Donor vector for 3' FLAG tag of human ELK1_iso1 |
| 135738 | pFETCh_ONECUT2 | Donor vector for 3' FLAG tag of human ONECUT2 |
| 135739 | PX458_ZNF331_1 | Encodes gRNA for 3' target of human ZNF331 |
| 135740 | PX458_ZNF331_2 | Encodes gRNA for 3' target of human ZNF331 |
| 135741 | PX458_ZBTB21_1 | Encodes gRNA for 3' target of human ZBTB21 |
| 135742 | PX458_ZBTB21_2 | Encodes gRNA for 3' target of human ZBTB21 |
| 135743 | PX458_CIC_iso1_1 | Encodes gRNA for 3' target of human CIC_iso1 |
| 135744 | PX458_CIC_iso1_2 | Encodes gRNA for 3' target of human CIC_iso1 |
| 135745 | PX330_MLLT10_iso1_1 | Encodes gRNA for 3' target of human MLLT10_iso1 |
| 135746 | PX330_ZBTB7B_1 | Encodes gRNA for 3' target of human ZBTB7B |
| 135747 | PX330_ZBTB7B_2 | Encodes gRNA for 3' target of human ZBTB7B |
| 135748 | PX330_ZNF264_iso1_1 | Encodes gRNA for 3' target of human ZNF264_iso1 |
| 135749 | PX330_MAF1_iso1_1 | Encodes gRNA for 3' target of human MAF1_iso1 |
| 135750 | PX330_MAF1_iso1_2 | Encodes gRNA for 3' target of human MAF1_iso1 |
| 135751 | PX330_MED13_iso1_1 | Encodes gRNA for 3' target of human MED13_iso1 |
| 135752 | PX458_FOXM1_iso1_1 | Encodes gRNA for 3' target of human FOXM1_iso1 |
| 135753 | PX458_FOXM1_iso1_2 | Encodes gRNA for 3' target of human FOXM1_iso1 |
| 135754 | PX330_ZNF503_1 | Encodes gRNA for 3' target of human ZNF503 |
| 135755 | PX330_ZNF503_2 | Encodes gRNA for 3' target of human ZNF503 |
| 135756 | PX330_E2F4_iso1_1 | Encodes gRNA for 3' target of human E2F4_iso1 |
| 135757 | PX330_ELK1_iso1_1 | Encodes gRNA for 3' target of human ELK1_iso1 |
| 135758 | PX330_ELK1_iso1_2 | Encodes gRNA for 3' target of human ELK1_iso1 |
| 135759 | PX330_ONECUT2_1 | Encodes gRNA for 3' target of human ONECUT2 |
| 135760 | PX330_ONECUT2_2 | Encodes gRNA for 3' target of human ONECUT2 |