A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. PubMed Article
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68283 | SepOTSβ | Phosphoserine orthogonal translation system |
| 68284 | SepOTSγ | Phosphoserine orthogonal translation system |
| 68285 | SepOTSδ | Phosphoserine orthogonal translation system |
| 68286 | SepOTSε | Phosphoserine orthogonal translation system |
| 68287 | SepOTSζ | Phosphoserine orthogonal translation system |
| 68288 | SepOTSη | Phosphoserine orthogonal translation system |
| 68289 | SepOTSθ | Phosphoserine orthogonal translation system |
| 68290 | SepOTSι | Phosphoserine orthogonal translation system |
| 68291 | SepOTSκ | Phosphoserine orthogonal translation system |
| 68292 | SepOTSλ | Phosphoserine orthogonal translation system (preferred variant) |
| 68294 | SepOTSν | Phosphoserine orthogonal translation system |
| 68295 | GFP E17TAG | E17TAG GFP reporter |
| 68296 | GFP S2TAG | S2TAG GFP reporter |
| 68297 | GFP Q157TAG | Q157TAG GFP reporter |
| 68298 | GFP S2TAG/E17TAG | S2TAG/E17TAG GFP reporter |
| 68299 | GFP E17TAG/Q157TAG | E17/Q157 TAG GFP reporter |
| 68300 | MBP-MEK1 | N-terminal MBP tagged human MEK1 gene for bacterial expression |
| 68301 | MBP-MEK1 S218TAG | N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression |
| 68302 | MBP-MEK1 S222TAG | N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression |
| 68305 | Beta lactamase S68TAG | Beta lactamase S68TAG gene |
| 68306 | Strain: C321.ΔA | Genomically recoded strain of E. coli (no genomic TAG) |
| 69118 | E17TAG GFP zeo resistance | For E17TAG GFP reporter expression in non-zeocin resistant strains |
| 198598 | SupD v2 | Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine. |